| Conditions: |
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Memory Disorders - Sleep Disorder |
| Purpose: |
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Background:
- The brain needs sleep to function normally, but the purpose of sleep is not understood.
Brain activity decreases during sleep, so it may be that sleep is important to
maintain, repair, or reorganize brain cells. In animals, the formation of brain
proteins increases during sleep, and the same thing may happen in humans.
- There is also evidence that learning and memory are helped by sleep, and that the
synthesis of proteins in the brain are involved.
Objectives:
- To examine the formation of proteins in the brain while people are awake, deprived of
sleep, and during sleep.
- To look at the formation of proteins in the brain while awake or asleep and following
learning a task.
Eligibility:
- Healthy volunteers between 18 and 28 years of age.
- Volunteers must not have psychiatric, neurologic, or sleep disorders or certain types
of vision problems, and must be able to undergo imaging studies.
Design:
- Study Part I (protein formation in waking, sleep deprivation, and sleep):
- Participants will wear an actigraph (a unit to record motor activity) for 2 weeks prior
to admission.
- Participants will have physical and psychological examinations, along with a blood
sample.
- After admission participants will have three positron emission tomography (PET) scans
to study protein formation and one magnetic resonance imaging (MRI) scan over the
course of two days.
- Participants may be asked to stay awake for as long as 20 hours and will be monitored
throughout.
- Participants will be able to sleep overnight after they complete the required scans and
monitoring, and will be discharged the following morning.
- Study Part II (protein formation in waking and sleep combined with a learning task):
- Participants will wear an actigraph (a unit to record motor activity) for 2 weeks prior
to admission.
- Participants will have physical and psychological examinations, along with a blood
sample.
- After admission participants may be asked to stay awake for as long as 20 hours and
will be monitored throughout.
- The next morning, participants will be trained to perform a computerized visual
discrimination task, and will be tested 8 hours later (after sleep or after remaining
awake) on the visual discrimination task.
- Some participants may have PET and MRI scans as part of the study.
- Participants will be able to sleep overnight after they complete the required tests and
scans, and will be discharged the following morning.
- Participants will receive financial compensation for their participation in these
studies.
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| Study Summary: |
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The importance of sleep is widely appreciated, but the actual function of sleep remains
unknown. Sleep occurs in much of the animal kingdom, in all mammals and birds and even in
some lower forms. Sleep deprivation impairs brain function, and in rats, total sleep
deprivation for 2-3 weeks results in death. One hypothesized role of sleep is for
restoration and reorganization of neuronal circuits. There is some indirect evidence that
during sleep, when cerebral energy requirements are reduced, cell resources are diverted to
protein synthesis for the restoration of structure and function. The objectives of the
present study are: 1) to further define the relationship between regional rates of cerebral
protein synthesis (rCPS) and sleep and 2) to ascertain whether sleep-dependent visual
learning during slow wave sleep (SWS) results in increases in rCPS in the primary visual
cortex. We propose to use a novel positron emission tomography (PET)-based technique to
quantify regional rates of cerebral protein synthesis (rCPS) in young, adult, healthy
volunteers. The first objective will be addressed in Part I in which we will study 15
subjects under the following three conditions: 1) awake and sleep-sated, 2) awake and
sleep-deprived, and 3) during SWS after sleep-deprivation. The second objective will be
addressed in Part II in which we will assess the relationship between rCPS and
sleep-dependent visual learning on a retinotopically specific task. Each participant will
serve as his own control by comparing the trained primary visual cortex hemisphere with the
untrained hemisphere to which comparable visual information is presented but without
learning. In Part II we will study two groups of 15 subjects each: 1) One group will be
studied during SWS following the training session; 2) The second group will be studied at
the same interval following the training session but awake. Subjects will be monitored with
polysomnography to identify the stages of sleep. We anticipate that the results of Part I
will identify changes in rCPS in specific brain regions which are characteristic of SWS and
results of Part II may reveal relationships between rCPS and memory consolidation during
SWS.
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| Criteria: |
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- INCLUSION CRITERIA:
Healthy male and female volunteers who have no present or past diagnosis of neurologic,
medical or psychiatric conditions which may confound either learning trails, normal sleep
patterns or the ability to undergo sleep deprivation.
Healthy male and female subjects, 18-28 years of age, who do not meet any exclusion
criteria, with self-reported normal sleep patterns (i.e., 6-9 h per night) and no major
sleep disruptions during the four weeks prior to evaluation will be considered for
inclusion in the study.
EXCLUSION CRITERIA INCLUDE A HISTORY OF, OR CURRENT:
1. chronic medical condition which is a contraindication for PET or MRI scanning,
2. past or present diagnosis of psychiatric conditions (DSM-IV criteria) (many
conditions (e.g. depression), may confound performance on learning trails or be
associated with baseline sleep abnormalities),
3. chronic/degenerative/acquired neurologic disorder, (4) family history of genetically
transmissible neurologic disorder,
(5) sleep disorders or medical conditions associated with chronically disordered sleep
which may confound performance on learning trails or interfere with the sleep requirements
of this study,
(6) visual impairments which may confound performance on learning trails,
(7) claustrophobic subjects,
(8) subjects who meet the above inclusion criteria, but are unable to cooperate with the
requirements of the study (e.g. refusal to wear actigraphs or maintain 10 hours time in
bed, reported difficulty sleeping away from home or on their backs).
(9) Subjects with chronic indicated or non-indicated use of any medications which
interfere with sleep architecture and/or learning trails will be excluded. Generally, we
will prohibit the use of medications/agents (e.g. anti-histamines, benadryl, melatonin,
cigarettes, chocolate, coffee, tea, caffeine drinks etc.), which have significant CNS
penetration, are alerting, and/or disrupt physiologic sleep-wake cycles or sleep
architecture, for the 72 hrs immediately preceding presentation for the study. To
acclimatize subjects, we will encourage patients to discontinue or minimize the use of
these agents/medications (e.g. less than or equal to 1 cup of coffee/day) at the time of
initial screening (at the same time as actigraphy application, approximately 2 wks prior
to the study).
Similarly, we ask subjects to minimize alcohol use for the 2 wks prior to the study and
will discontinue alcohol use for the 72 hrs immediately preceding the study.
(10) Subjects who have used illicit drugs (marijuana, cocaine, heroin, etc.) within the
immediate 2 wks preceding the study.
(11) Female subjects will be excluded if either clinical history is suspicious for, or
laboratory evaluation is consistent with pregnancy.
(12) Subjects unwilling to undergo HIV testing, unless enrolling in Part IIa of the study.
(13) Subjects who test HIV positive.
(14) Patients with para- and/or ferro-magnetic prosthesis/implants/fragments in their body
will be excluded from the study.
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| NCT ID: |
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NCT00884702 |
| Primary Contact: |
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Principal Investigator
Carolyn B Smith, Ph.D.
National Institute of Mental Health (NIMH)
Carolyn B Smith, Ph.D.
Phone: (301) 451-8995
Email: beebe@mail.nih.gov
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| Backup Contact: |
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N/A |
| Location Contact: |
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Bethesda, Maryland 20892
United States
For more information at the NIH Clinical Center contact Patient Recruitment and Public Liaison Office (PRPL)
Phone: 800-411-1222
Email: prpl@mail.cc.nih.gov
Site Status: Recruiting |