View Clinical Trial (Medical Research Study)

Genetics of Peripheral Artery Genomics - NCT00615121-27710(Clinical Trial 202576)



ClinicalConnection.com has recently undergone an update and this page may no longer be up-to-date. Please Search For Clinical Trials to view the most current clinical trials listings.


Signup
City:  Durham
State:  
NC
Zip Code: 27710
Conditions: Peripheral Artery Disease
Purpose: Peripheral artery disease is a disease that contributes to significant morbidity and mortality of millions of Americans yearly. Very little is known about the pathophysiology of atherosclerosis in peripheral artery disease. We plan to collect peripheral arteries from patients undergoing amputation for end stage peripheral arterial occlusive disease. By extracting the RNA from these arteries and comparing them with RNA expression from normal arteries, we hope to have a better understanding of the pathophysiology of atherosclerosis in this setting. We aim to prove the hypothesis that a novel gene expression pattern can be discovered by the successful extraction of RNA from plaques from human peripheral arteries.
Study summary: Patients scheduled to undergo lower extremity amputation will be consented for extraction of plaque from the carotid artery or arterial tissue from the amputated limbs. The surgeon will perform the amputation, then the harvest team will collect arterial tissue from amputated limbs. These samples will be immersed in RNALater solution to preserve the integrity of the RNA. Tissues will stored at -80oC in the RNALater until the RNA is later extracted using a standardized protocol. The RNA will be examined for quality using an Agilent bioanalyzer, and will then be processed on Affymetrix U133A chips for gene expression data. The data will be analyzed with the MATLAB software package.
Criteria: Inclusion Criteria: - Patients undergoing amputations for peripheral artery disease - Patients undergoing free fibular transfer Exclusion Criteria: - those that are not able to consent to the study
Study is available at: Duke University Medical Center
Durham, NC 27710
United States

Primary Contact:
Bantayehu Sileshi, MD
Email: bantayehu.sileshi@duke.edu
Phone: (919) 668-6112
If you are interested in this clinical trial please use the contact information above. If you would like to get additional information about this clinical trial please visit ClinicalTrials.gov.
Trials Alerts: If you would like to be notified of new clinical trials as they become available please register for a free account.
Data Source: ClinicalTrials.gov
Date Processed: March 22, 2011
Modifications to
this listing:		 Only selected fields are shown, please use the link above to view all information about this clinical trial.