Expired Study
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Omaha, Nebraska 68198


RATIONALE: Giving low doses of chemotherapy and total-body irradiation before a donor peripheral blood stem cell transplant helps stop the growth of cancer cells. It may also stop the patient's immune system from rejecting the donor's stem cells. The donated stem cells may replace the patient's immune cells and help destroy any remaining cancer cells (graft-versus-tumor effect). Sometimes the transplanted cells from a donor can also make an immune response against the body's normal cells. Removing the T cells from the donor cells before transplant and giving cyclosporine and mycophenolate mofetil after transplant may stop this from happening. PURPOSE: This phase I/II trial is studying the side effects of giving a donor stem cell transplant after pentostatin and total-body irradiation and to see how well it works in treating patients with hematological cancer.

Study summary:

OBJECTIVES: Primary - To determine the safety of pentostatin and low-dose total body irradiation followed by T-cell-reduced unrelated donor peripheral blood stem cell transplantation, in terms of regimen-related toxicity, in patients with hematological malignancies. - To evaluate the efficacy of this regimen, measured as engraftment rate and establishment of donor hematopoietic chimerism, in these patients. Secondary - To determine the incidence of acute and chronic graft-versus-host disease in patients treated with this regimen. OUTLINE: - Reduced-intensity preparative regimen: Patients receive pentostatin IV over 30 minutes once daily on days -10 to -8 and undergo low-dose total-body irradiation on day -1. - Unrelated donor peripheral blood stem cell transplantation (PBSCT): Patients undergo T-cell-reduced donor PBSCT on day 0. - Graft-versus-host disease (GVHD) prophylaxis: Patients receive cyclosporine IV over 2 hours twice daily on days -1, 0, and 1 and then orally twice daily on days 2-70 followed by a taper in the absence of GVHD. Patients also receive oral mycophenolate mofetil twice daily on days 0-27 followed by a taper. Patients undergo bone marrow aspirate and biopsies and blood sample collection periodically for laboratory studies. Samples are analyzed for cytokines (i.e., IL-6, TNF-γ, IL-1β, and IL-10) by ELISA; phenotypic, molecular, and functional analysis of immunologic reconstitution markers (i.e., PHA, IL-2, IL-4, IL-10, IL-12, Fas, FasL, TNF, TGF-β, and IFN-γ) by flow cytometry; and cytogenetics by FISH. After completion of study treatment, patients are followed periodically.


DISEASE CHARACTERISTICS: - Diagnosis of a confirmed hematological malignancy that has relapsed or is at high risk for relapsing, including any of the following: - Acute myeloid leukemia (AML) meeting any of the following criteria: - Antecedent hematologic disorder - Therapy related - Primary induction failure - In first complete remission (CR1) with poor-risk cytogenetics, as defined by the following: - del(5q)/-5 - del(7q)/-7 - abn(3q) - t(6;9) - del(20q) - del(17p) - +13 - Complex karyotype - t(9;22) = 11q23 rearrangement - In second complete remission (CR2) or greater - Acute lymphoblastic leukemia meeting any of the following criteria: - In CR1 with WBC > 50,000/mm³ at diagnosis - In CR1 with poor-risk cytogenetics (i.e., t[9;22], t[1;19], t[4;11]) AND meets at least 1 of the following criteria: = 19-75 years of age AND received prior high-dose chemotherapy, total-body irradiation (TBI), or a radiation dose that precludes administration of 12 Gy of TBI = 50-75 years of age = 19-75 years of age with hematopoietic stem cell transplantation (HSCT) comorbidity index ≥ 3 - CNS or testicular involvement at diagnosis - No CR within 4 weeks of initial treatment - Primary induction failure - In CR2 or greater - Myelodysplastic syndromes meeting the following criteria: - Intermediate-2 or high-risk category as determined by International Prognostic Scoring System - Not considered a candidate for intensive or standard chemotherapy or HSCT - Chronic myelogenous leukemia meeting any of the following criteria: - First chronic phase AND < 40 years of age - First chronic phase AND no hematologic response after 3 months of imatinib mesylate therapy - First chronic phase AND never achieved a complete cytogenetic response during imatinib mesylate therapy - First chronic phase AND loss of previously documented response - Accelerated phase - Blast crisis phase - Chronic myeloproliferative disorder (i.e., polycythemia vera, essential thrombocythemia, myelofibrosis) - Bone marrow blasts > 5% and/or other evidence of progression to acute leukemia - Chronic myelomonocytic leukemia - Severe aplastic anemia - Failed prior antithymocyte globulin and cyclosporine immunosuppressive therapy - Mantle cell lymphoma meeting any of the following criteria: - In CR1 - In first partial remission (PR1) - In CR 2 or greater - In second PR (PR2) or greater - Indolent non-Hodgkin lymphoma OR chronic lymphocytic leukemia meeting either of the following criteria: - In CR 2 or greater - In PR 2 or greater - Lymphoblastic lymphoma - In CR1 or greater - Must have minimal residual disease as defined by either of the following: - No more than 5% blasts in blood and/or bone marrow (in patients with acute leukemia/MDS) - No bulky adenopathy (> 5 cm masses) and/or < 20% bone marrow involvement by lymphoma (in patients with lymphoma) - No progressive disease within 8 weeks of most recent prior therapy OR within 12 weeks of prior autologous HSCT - No active CNS malignancy (i.e., known positive CSF cytology or parenchymal lesions visible by CT scan or MRI) - HLA-matched unrelated peripheral blood stem cell donor available - Meets the University of Nebraska Medical Center's or the National Marrow Donor Program's criteria for donors - Matched at 7/8 or 8/8 HLA-A, B, C, or DRβ1 loci by molecular typing - If match is not at allele level, suitability for donation requires discussion with and approval by the principal investigator - Not an identical twin PATIENT CHARACTERISTICS: - Karnofsky performance status 60-100% - Creatinine clearance ≥ 55 mL/min - Total bilirubin ≤ 2 times upper limit of normal (ULN) (unless due to Gilbert's disease or malignancy) - ALT and AST ≤ 4 times ULN - DLCO ≥ 40% - FEV1/FVC ratio ≥ 50% of predicted - Cardiac ejection fraction ≥ 40% - Not pregnant or nursing - Fertile patients must use effective contraception - Not receiving supplementary continuous oxygen - No NYHA grade II-IV cardiac disease - HIV negative - No evidence of active hepatitis B (i.e., positive HBsAg and/or positive HBeAg or high copy number on quantitative RNA testing) or hepatitis C - No active uncontrolled infection or immediate life-threatening condition - No uncontrolled medical illnesses (e.g., uncontrolled systemic hypertension or diabetes) PRIOR CONCURRENT THERAPY: - See Disease Characteristics - Prior cytoreductive chemotherapy or irradiation to areas of bulky disease allowed, as determined by the primary physician in consultation with the study investigators - No other concurrent anti-tumor therapy



Primary Contact:

Principal Investigator
Robert Bociek, MD
University of Nebraska

Backup Contact:


Location Contact:

Omaha, Nebraska 68198
United States

There is no listed contact information for this specific location.

Site Status: N/A

Data Source: ClinicalTrials.gov

Date Processed: October 09, 2019

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